![]() ![]() IUI, IVF/ICSI and FET outcomes were consistent with unimpaired sperm function. Conclusionsįlow cytometric sorting of human sperm shifted the X:Y sperm ratio. IUI, IVF/ICSI, and FET clinical pregnancy rates were 14.7%, 30.8%, and 32.1%, respectively clinical miscarriage rates were 15.5%, 10.2%, and 12.7%. For babies born, 93.5% were females and 85.3% were males after sorting for X- and Y-bearing sperm, respectively. Seventy-three percent of sorts were for girls. Sorted specimens averaged 87.7 ± 5.0% X-bearing sperm after sorting for X and 74.3 ± 7.0% Y-bearing sperm after sorting for Y. Measures of effectiveness were the percentage of X- and Y-bearing sperm in sorted samples, determined by fluorescence in situ hybridization, sex of babies born, IVF/ICSI fertilization- and cleavage rates, and IUI, IVF/ICSI, FET pregnancy rates and miscarriage rates. Sorted sperm were used for IUI (n = 4448) and IVF/ICSI (n = 2957). Fresh or frozen-thawed semen was processed and recovered sperm were stained with Hoechst 33342 and sorted by flow cytometry (n = 7718) to increase the percentage of X-bearing sperm (n = 5635) or Y-bearing sperm (n = 2083) in the sorted specimen. After meeting inclusion criteria, married couples (n = 4993) enrolled to reduce the likelihood of sex-linked or sex-limited disease in future children (n = 383) or to balance the sex ratio of their children (n = 4610). This prospective, observational cohort study of the series of subjects treated with flow cytometrically sorted human sperm was conducted at investigational sites at two private reproductive centers. Herein we report the effectiveness of flow cytometric sorting of human sperm and its use in human ART procedures. Y-chromosome-bearing sperm enables sorting and collection of samples enriched in either X- or Y-bearing sperm for use to influence the likelihood that the offspring will be a particular sex. Differential fluorescence emitted by stained X- vs. Flow cytometric sorting can be used to separate sperm based on sex chromosome content. ![]()
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